Research Abstract

Research Abstract

I am the Director of the CU-Boulder Stem Cell Research and Technology Resource Center, which is a campus-wide shared facility with the mission of promoting interdisciplinary research utilizing human induced pluripotent stem cells (iPSCs).

During my PhD at UC Santa Barbara in the laboratory of Dennis O. Clegg, I specialized in stem cell biology (primarily utilizing human iPSCs and hESCs) with a focus on the effects of the extracellular matrix (ECM) on the adhesion, proliferation, and differentiation of stem cells. These efforts were aimed at ultimately treating patients with age-related macular degeneration (AMD) by developing an efficient method to generate retinal pigmented epithelium (RPE) from iPSCs and hESCs and creating an implantable scaffold system for patient transplantation of RPE cells. As a result, treatment strategies building upon my efforts are now nearing clinical trials. In my first postdoctoral position at the CU Denver Anschutz Medical Campus in the joint lab of Luisa Mestroni and Matthew R.G. Taylor, I gained valuable experience and knowledge in the areas of generating patient-specific disease models through differentiation of patient-derived induced pluripotent stem cells (iPSCs) into cardiomyocytes, as well as designing and undertaking high-throughput drug screening assays using these patient-derived cells. I went on to become a postdoctoral researcher at CU-Boulder in the lab of Prof. Thomas Cech, where I researched the epigenetic mechanisms by which cancer cells become immortal through reactivation of telomerase.


19. Begay, R.L., Graw, S.L., Sinagra, G., Slavov, D.B., Jones, K.L., Gowan, K., Rowland, T.J., Wartchow, E.P., Brun, F., Asamaki, A., Saffitz, J.E., Sweet, M., Garrity, D.M., Gigli, M., Mestroni, L., Taylor, M.R.G. (in preparation) Arrhythmogenic Phenotype is Associated with Familial Dilated Cardiomyopathy Caused by Novel Filamin C Truncation Mutations.

18. Rowland, T.J., Graw, L.S., Sweet, M.E., Gigli, M., Taylor, M.R.G., Mestroni, L. (accepted) Obscurin Variants in Patients with Left Ventricular Noncompaction. Journal of the American College of Cardiology.

17. Daniel, J., Fetter, L., Jett, S., Rowland, T.J., Bonham, A.J. (accepted) Electrochemical Aptamer Scaffold Biosensors for Detection of Botulism and Ricin Proteins, in Methods in Molecular Biology, Springer.

16. Begay, R.L., Tharp, C.A., Martin, A., Graw, S.L., Sinagra, G., Miani, D., Slavov, D.B., Rowland, T.J., Stafford, N., Sweet, M.E., Brun, F., Jones, K.L., Gowan, K., Mestroni, L., Garrity, D.M., Taylor, M.R.G. (accepted) FLNC Gene Splice Mutations Cause Dilated Cardiomyopathy. Journal of the American College of Cardiology: Basic to Translational Science.

15. Puggia, I., Merlo, M., Barbati, G., Rowland, T.J., Stolfo, D., Gigli, M., Ramani, F., Di Lenarda, A., Sinagra, G. (accepted) Natural History of Dilated Cardiomyopathy in Children. Journal of the American Heart Association.

14. Rowland, T.J., Mestroni, L., Taylor, M.R.G. (2016) Danon Disease: Dysregulation of Autophagy in a Multisystem Cardiomyopathy. Journal of Cell Science, 129(11):2135-43.

13. Puggia, I., Rowland, T.J., Mestroni, L. (accepted) Molecular and Cellular Mechanisms in Heart Failure, in Heart Failure in the Child and Young Adult: From Bench to Bedside, Elsevier.

12. Rowland, T.J., Clegg, D.O., Gamm, D.M. (accepted) Stem Cells and Cellular Therapy, in Stephen J. Ryan Retina, 6th edition, Elsevier.

11. Fetter, L., Richards, J., Daniel, J., Roon, L., Rowland, T.J., Bonham, A.J. (2015) Electrochemical Aptamer Scaffold Biosensors for Detection of Botulism and Ricin Toxins. Chemical Communications, 51:15137-15140.

10. Hossein, N., Zhang, L., Zhu, D., Chader, G., Falabella, P., Stefanini, F., Rowland, T.J., Clegg, D.O., Kashani, A., Hinton, D., Humayun, M. (2015) Stem Cell Based Therapies for Age-Related Macular Degeneration: The Promises and the Challenges. Progress in Retinal and Eye Research, 48: 1-39.

9. Clegg, D.O., Hikita, S.T., Buchholz, D.E., Rowland, T.J., Conti, L., Pennington, B., Croze, R., Leach, L., Tsie, M., and Johnson, L.V. (2013) Derivation of retinal pigmented epithelial cells from pluripotent stem cells, in Stem Cells Handbook, Second Edition, S. Sell, Ed., Springer.

8. Rowland, T.J., Blaschke, A.J., Buchholz, D.E., Hikita, S.T., Johnson, L.V., Clegg, D.O. (2013) Differentiation of Human Pluripotent Stem Cells to Retinal Pigmented Epithelium in Defined Conditions Using Purified Extracellular Matrix Proteins. Journal of Tissue Engineering and Regenerative Medicine, 7 (8): 642-653.

7. Rowland, T.J., Buchholz, D.E., Clegg, D.O. (2012) Pluripotent Human Stem Cells for the Treatment of Retinal Disease. Journal of Cellular Physiology, 227 (2): 457-466.

6. Rowland, T.J. (2011) Human Pluripotent Stem Cells and the Role of the Extracellular Matrix in Undifferentiated Growth and Differentiation to Retinal Pigmented Epithelium. Dissertation.

5. Rowland, T.J., Miller, L.M., Blaschke, A.J., Doss, E.L., Bonham, A.J., Hikita, S.T., Johnson, L.V., Clegg, D.O. (2010) Roles of Integrins in Human Induced Pluripotent Stem Cell Growth on Matrigel and Vitronectin. Stem Cells and Development, 19 (8): 1231-1240.

4. Clegg, D.O., Rowland, T.J. (2010) Dan E. Koshland, Jr. Proceedings of the American Philosophical Society, 154 (4): 478-483.

3. Buchholz, D.E., Hikita, S.T., Rowland, T.J., Friedrich, A.M., Hinman, C.R., Johnson, L.V., Clegg, D.O. (2009) Derivation of Functional Retinal Pigmented Epithelium from Induced Pluripotent Stem Cells. Stem Cells, 27 (10): 2427-2434.

2. Clegg, D.O., Buchholz, D., Hikita, S.H., Rowland, T.J., Hu, Q., Johnson, L.V. (2008) Retinal Pigment Epithelial Cells: Development in vivo and Derivation from Human Embryonic Stem Cells in vitro for Treatment of Age-Related Macular Degeneration, in Stem Cell Research and Therapeutics, Springer.

1. Hamilton, E.P., Dear, P.H., Rowland, T.J., Saks, K., Eisen, J.A., Orias, E. (2006) Use of HAPPY Mapping for the Higher Order Assembly of the Tetrahymena Genome. Genomics, 88: 443-451.

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